RNA preparation from cell lines, primary cultures, human and animal tissues. Quality control of RNA by Nano 6000 RNA lab chips kits and an Agilent 2100 Bioanalyzer. Indivumed offers comprehensive studies based on its high-quality biobank for gene expression analysis using e.g., Affymetrix arrays/Nimble Gene platform.
This includes:
- Preparation of comparative sample groups using Indivumed data base and biobank
- RNA preparation from cell lines, primary cultures, human and animal tissues. Quality control of RNA by Nano 6000 RNA lab chips kits and an Agilent 2100 Bioanalyzer.
- cDNA and cRNA preparations
- Photometric, fluorescence based and gel analytical (denaturating and non-denaturating) characterization and concentration determination of nucleic acids
(Biophotometer, Eppendorf/Fluostar Optima, BMG) - Bioinformatics and Biostatistics (including clinical data of indivuNET)
Validation of potential biomarkers by
- Quantitative RNA/DNA expression analysis with real-time PCR (96 well format; iCycler)
- Radioactive and non-radioactive (DIG system based) analysis of nucleic acids by Northern and Southern Blot techniques
- Amplification, cloning and expression of DNA/cDNA- fragments in various expression systems
Approx. 300 clinical data points from each patient sample offer a unique opportunity to obtain clinical meaningful results by using Indivumed’s data base indivuNET and bioinformatic and statistical tools. Indivumed’s capability to perform within its clinical network validation studies within a short period of time provides another advantage for customers when collaborating with Indivumed for biomarker identification and validation.
